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University of Utah

Department of Biochemistry

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Opportunities for Students

Graduate students can join the lab through the programs in Molecular Biology or Biological Chemistry. We welcome rotation students.

Papers

Jacobsen, M.T., Erickson, P.W., Kay, M.S. (2017) Aligator: A computational tool for optimizing total chemical synthesis of large proteins. Bioorg Med Chem., 25: 4946-4952.

 

Trettin, K.D., Sinha, N.K., Eckert, D.M., Apple, S.E., Bass, B.L. (2017) Loquacious-PD facilitates Drosophila Dicer-2 cleavage through interactions with the helicase domain and dsRNA. Proc. Natl. Acad. Sci. USA, 114: E7939-E7948.

 

Jacobsen, M.T., Petersen, M.E., Ye, X., Galibert, M., Lorimer, G.H., Aucagne, V., Kay, M.S. (2016) A helping hand to overcome solubility challenges in chemical protein synthesis. J. Am. Chem. Soc., 138: 11775-82.

 

Petersen, M.E., Jacobsen, M.T., Kay, M.S. (2016) Synthesis of tumor necrosis factor α for use as a mirror-image phage display target. Org. Biomol. Chem., 14: 5298-303.

 

Neti, S.S., Eckert, D.M., Poulter, C.D. (2016) Construction of functional monomeric Type 2 Isopentenyl Diphosphate:Dimethylallyl Diphosphate Isomerase. Biochemistry, 55: 4229-38.

 

Clinton, T.R., Weinstock, M.T., Jacobsen, M.T., Szabo-Fresnais, N., Pandya, M.J., Whitby, F.G., Herbert, A.S., Prugar, L.I., McKinnon, R., Hill, C.P., Welch, B.D., Dye, J.M., Eckert, D.M., Kay, M.S. (2015) Design and characterization of ebolavirus GP prehairpin intermediate mimics as drug targets. Protein Sci., 24:446-63. *2016 Protein Science Best Paper Award Winner

 

Zhao, H.,  Ghirlando, R., Alfonso, C.,  Arisaka, F., Attali, I., Bain, D.L., Bakhtina, M.M., Becker, D.F., Bedwell, G.J., Bekdemir, A., Besong, T.M.D., Birck, C., 12, Brautigam, C.A., Brennerman, W., Byron, O., Bzowska, A., Chaires, J.B., Chaton, C.T., Cölfen, H., Connaghan, K.D., Crowley, K.A., Curth, U., Daviter, T., Dean, W.L., Díez, A.I., Ebel, C., Eckert, D.M., Eisele, L.E., Eisenstein, E., England, P., Escalante, C., Fagan, J.A., Fairman, R., Finn, R.M., Fischle, W., García de la Torre, J., Gor, J., Gustafsson, H., Hall, D., Harding, S.E., Hernández Cifre, J.G., Herr, A.B., Howell, E.E., Isaac, R.S., Jao, S., Jose, D., Kim, S., Kokona, B., Kornblatt, J.A., Kosek, D., Krayukhina, E., Krzizike, D., Kusznir, E.A., Kwon, H., Larson, A., Laue, T.M., LeRoy, A., Leech, A.P., Lilie, H., Luger, K., Luque-Ortega, J.R., Ma, J., May, C.A., Maynard, E.L., Modrak-Wojcik, A., Mok, Y., Mücke, N.,  Nagel-Steger, L., Narlikar, G.J., Noda, M., Nourse, A., Obsil, T., Park, C.K., Park, J., Pawelek, P.D., Perdue, E.E., Perkins, S.J., Perugini, M.A., Peterson, C.L., Peverelli, M.G., Piszczek, G., Prag, G., Prevelige, P.E., Raynal, B.D.E., Rezabkova, L., Richter, K., Ringel, A.E., Rosenberg, R., Rowe, A.J., Rufer, A.C., Scott, D.J., Seravalli, J.G., Solovyova, A.S., Song, R., Staunton, D., Stoddard, C., Stott, K., Strauss, H.M., Streicher, W.W., Sumida, J.P., Swygert, S.G., Szczepanowski, R.H., Tessmer, I., Toth IV, R.T., Tripathy, A., Uchiyama, S., Uebel, S.F.W., Unzai, S., Vitlin Gruber, A., von Hippel, P.H., Wandrey, C., Wang, S., Weitzel, S.E., Wielgus-Kutrowska, B., Wolberger, C., Wolff, M., Wright, E., Wu, Y., Wubben, J.M., Schuck, P.* (2015) A Multilaboratory Comparison of Calibration Accuracy and the Performance of External References in Analytical Ultracentrifugation. Plos One, 10(5), e0126420.

 

Weinstock, M.T., Jacobsen, M.T. and Kay, M.S. (2014) Synthesis and folding of a mirror-image enzyme reveals ambidextrous chaperone activity. Proc. Natl. Acad. Sci. USA., 111:11679-84.

 

Ren, J., Pei-Chen Lin, C., Pathak, M.C., Temple, B.R., Nile, A.H., Mousley, C.J., Duncan, M.C., Eckert, D.M., Leiker, T.J., Ivanova, P.T., Myers, D.S., Murphy, R.C., Brown, H.A.,Verdaasdonk, J., Bloom, K.S., Ortlund, E.A., Neiman, A.M., and Bankaitis, V.A. (2014) A Phosphatidylinositol Transfer Protein Integrates Phosphoinositide Signaling with Lipid Droplet Metabolism to Regulate a Developmental Program of Nutrient Stress-Induced Membrane Biogenesis. Mol Biol. Cell, 25:712-27.

 

Monroe, N., Han, H., Gonciarz, M.D., Eckert, D.M., Karren, M.A., Whitby, F.G., Sundquist, W.I.,  and Hill, C.P. (2014) The oligomeric state of the active Vps4 AAA ATPase. J. Mol. Biol., 426:510-25.

 

Mesquita, P.M., Srinivasan, P., Johnson, T.J., Rastogi, R., Evans-Strickfaden, T., Kay, M.S., Buckheit, K.W., Buckheit, R.W. Jr., Smith, J.M., Kiser, P.F. and Herold, B.C. (2013) Novel preclinical models of topical PrEP pharmacodynamics provide rationale for combination of drugs with complementary properties. Retrovirol., 10:113.

 

Koirala, S., Guo, Q., Kalia, R., Bui, H.T., Eckert, D.M., Frost, A., and Shaw, J.M. (2013) Interchangeable adaptors regulate mitochondrial dynamin assembly for membrane scission. Proc. Natl. Acad. Sci. USA, 110: E1342-51.

 

Pang, H.B., Hevroni, L., Kol, N., Eckert, D.M., Tsvitov, M., Kay, M.S., and Rousso, I. (2013) Virion stiffness regulates immature HIV-1 entry. Retrovirol., 10:4.

 

Weinstock, M.T., Francis, J.N., Redman, J.S., and Kay, M.S. (2012) Protease-Resistant Peptide Design – Empowering Nature’s Fragile Warriors Against HIV. Peptide Science, 98: 431-42.

 

Stadtmueller, B.M., Kish-Trier, E., Ferrell, K., Petersen, C.N., Robinson, H., Myszka, D.G., Eckert, D.M., Formosa, T., and Hill C.P. (2012) Structure of a proteasome Pba1-Pba2 complex: implications for proteasome assembly, activation, and biological function. J. Biol Chem. 287: 37371-82.

 

Francis, J.N., Redman, J.S., Eckert, D.M. and Kay, M.S. (2012) Design of a modular tetrameric scaffold for the synthesis of membrane-localized D-peptide inhibitors of HIV-1 entry.  Bioconjugate Chem. 23: 1252-1258.

 

Orsak, T., Smith, T.L., Eckert, D., Lindsley, J.E., and Rutter, J. (2011) Revealing the allosterome: systematic identification of metabolite-protein interactions. Biochem. 51: 225-32.

 

Denton, P.W., Othieno, F., Martinez-Torres, F., Zou, W., Krisko, J.F., Fleming, E., Zein, S., Powell, D.A., Wahl, A, Kwak, Y.T., Welch, B.D., Kay, M.S., Payne, D.A., Gallay, P., Appella, E., Estes, J.D., Lu, M. and Garcia, J.V. (2011) One percent tenofovir applied topically to humanized BLT mice and used according to the CAPRISA 004 experimental design demonstrates partial protection from vaginal HIV infection, validating the BLT model for evaluation of new microbicide candidates. J Virol. 85:7582-93.

 

Welch, B.D., Francis, J.N., Redman, J.S., Paul, S., Weinstock, M.T., Reeves, J.D., Lie, Y.S., Whitby F.G., Eckert, D.M., Hill, C.P., Root, M.J., and Kay, M.S. (2010) Design of a Potent D-peptide HIV-1 Entry Inhibitor with a Strong Barrier to Resistance. J Virol., 84: 11235-44.

 

Kol N., Tsvitov M., Hevroni L., Wolf S. G., Pang H. B., Kay M. S., and Rousso I. (2010) The effect of purification method on the completeness of the immature HIV-1 Gag shell. J Virol Methods. 169:244-7.

 

Eckert, D.M. and Kay, M.S. (2010) Stalking Influenza. Proc. Natl. Acad. Sci. USA 107: 13563-13564.

 

Koirala, S., Bui, H.T., Schubert, H.L., Eckert, D.M., Hill, C.P., Kay, M.S. and Shaw, J.M. (2010) Molecular architecture of a dynamin adaptor: implications for assembly of mitochondrial fission complexes. J Cell Biol. 191: 1127-1139.

 

Schubert, H.L., Zhai, Q., Sandrin, V., Eckert, D.M., Garcia-Maya, M., Saul, L., Sundquist, W.I., Steiner, R.A., and Hill, C.P. (2010) Structural and Functional Studies on the Extracellular Domain of BST2/Tetherin in Reduced and Oxidized Conformations. Proc. Natl. Acad. Sci. USA, 107: 17951-6.

 

Bajorek, M., Schubert, H.L., McCullough, J., Langelier, C., Eckert, D.M., Stubblefield, W-M.B., Uter, N.T., Hill, C.P., and Sundquist, W.I. (2009) Structural basis for ESCRT-III protein autoinhibition. Nat. Struct. Mol. Biol. 16: 754-762.

 

Kim, S., and Kay M. S. (2008) Peptide Mimic of the HIV envelope gp120-gp41 interface. J Mol Biol. 376: 786-797.

 

Eckert, D.M., Shi, Y., Kim, S., Welch, B.D., Kang, E., Poff, E.S., and Kay, M.S. (2008) Characterization of the steric defense of the HIV-1 gp41 N-trimer region. Protein Sci. 17: 2091-2100.

 

Gonciarz, M.D., Whitby, F.G., Eckert, D.M., Kieffer, C., Heroux, A., Sundquist, W.I., and Hill, C.P. (2008) Biochemical and structural studies of yeast Vps4 oligomerization. J. Mol. Biol. 384: 878-895.

 

Langelier, C.R., Sandrin, V., Eckert, D.M., Christensen, D.E., Chandrasekaran, V., Alam, S.L., Aiken, C., Olson, J.C., Kar, A.K., Sodroski, J.G., and Sundquist, W.I. (2008) Biochemical characterization of a recombinant TRIM5α protein that restricts HIV-1 replication. J. Virol. 82: 11682-11694.

 

Welch, B.W., VanDemark, A.P., Hill, C.P., and Kay, M.S. (2007) Potent D-peptide inhibitors of HIV-1 entry. Proc. of the Natl. Acad. Sci. USA 104: 16828-33.

 

Kol, N., Shi, Y., Tsvitov, M., Barlam, D., Shneck, R.Z., Kay, M.S. & Rousso, I. (2007) A stiffness switch in Human Immunodeficiency Virus. Biophys. J. 92: 1777-1783.

 

Morita, E., Sandrin, V., Alam, S.L., Eckert, D.M., Gygi, S.P. and Sundquist, W.I. (2007) Identification of human MVB12 proteins as ESCRT-I subunits that function in HIV budding. Cell Host Microbe 2: 41-53.

 

Parker, G.S., Eckert D.M., and Bass, B.L. (2006) RDE-4 preferentially binds long dsRNA and its dimerization is necessary for cleavage of dsRNA to siRNA. RNA 12: 807-818.

 

Azmi, I., Davies, B., Dimaano, C., Payne, J., Eckert D., Babst, M., and Katzmann, D.J. (2006) Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a conserved VSL region in Vta1. J. Cell Biol. 172: 705-717.

 

Hamburger, A.H., Kim, S., Welch, B.D., Kay M.S. (2005) Steric accessibility of the HIV-1 gp41 N-trimer region. J. Biol Chem. 280: 12567-72.

 

Stephens, O.M., Kim, S., Welch, B.D., Hodsdon, M.E., Kay, M.S., Schepartz, A. (2005) Inhibiting HIV fusion with a ß-peptide foldamer. J Am Chem Soc. 127: 13126-7.

 

Kay, M.S. (2003) Silent, but deadly – eliminating reservoirs of latent HIV. Trends Biotechnol. 21: 420-423.

 

Root, M.J., Kay, M.S., and Kim, P.S. (2001) Protein design of an HIV-1 entry iInhibitor. Science 291, 884-888.

 

Eckert, D.M. and Kim, P.S. (2001) Design of Potent Inhibitors of HIV-1 Entry from the gp41 N-Peptide Region. Proc. Natl. Acad. Sci. USA 98: 11187-11192.

 

Eckert, D.M., Malashkevich, V.N., Hong, L.H., Carr, P.A., and Kim, P.S. (1999) Inhibiting HIV-1 Entry: Discovery of D-Peptide Inhibitors that Target the gp41 Coiled-Coil Pocket. Cell  99: 103-115.

 

University of Utah, Department of Biochemistry, 15 N Medical Drive East RM 4100, Salt Lake City, UT 84112-5650 USA

Office: (801) 585-5021 | Lab: (801) 585-9512 | kay@biochem.utah.edu