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Carroll Lab Stocks

 

       
Box1: Important Stocks,Mapping Stocks, FLP+/- I-SceI Stocks      
Name Genotype Comments  
Important Stocks      
v; ry v; ry to work with ry+ vectors  
W1118 W1118 to work with w+ vectors  
c(1)DX C(1)D, yf / Fm6, yB / Y "attached X" females  
yw y,w from Kent  
Mapping Stocks      
v; Sco / Cy; ry v; Sco / Sm6,Cyo; ry Chromosome 2 balancer  
v; Sb / Tm6,Ubx v; eukor2,Sb / Tm6,Ubx Chromosome 3 balancer  
W1118; Sco / S2,Cyo W1118; Sco / S2,Cyo Chromosome 2 balancer  
W1118; Sb / Tm6,Ubx W1118; eukor2,Sb / Tm6,Ubx Chromosome 3 balancer  
FLP and FLP+I-SceI stocks      
W1118; FLP11 W1118; [ry+,70 FLP]11 / S2,Cyo FLP on 2, only balanced $  
W1118; [FLP11+I-Sce1] W1118; [ry+,70 FLP]11,[v+,70 I-Sce1]2b / Cy FLP+I-Sce1 on 2, from Yikang, hz live  
W1118; [FLP11+I-Sce1] W1118; [ry+,70 FLP]11,[v+,70 I-Sce1]2b FLP+I-Sce1 on 2, hz $  
  W1118; [FLP+I-SceI]2B/[ry+FLP][v+,I-SceI]23    
W1118; FLP23 W1118; [ry+,70 FLP]23,ry / Tm6, Ubx FLP on 3, only balanced $  
W1118; [FLP23+I-Sce1] W1118; [ry+,70 FLP]23,[v+,70 I-Sce1]4A / Tm3,Ser FLP+I-SceI on 3, from Yikang, hz live  
Other Stocks with FLP and I-SceI      
FLP11 v; [ry+,70 FLP]11 / Sm6,Cy; ry FLP on 2, from Yikang  
FLP23 v; [ry+,70 FLP]23,ry / Tm2,Ubx,ry FLP on 3, from Yikang  
W1118; 70 FLP 4A W1118; 70 FLP 4A FLP on 3, from Mary  
  v; [70 FLP][70 I-SceI]Sco / Sm6,Cy; ry FLP+I-SceI marked with Sco, from Diane  
W1118; [FLP+I-SceI]Sco W1118; [70 FLP][70 I-Sce1]Sco / S2,Cyo FLP+I-SceI marked with Sco  
W1118; hs FLP2B / S2,Cyo W1118; hs FLP2B / S2,Cyo FLP on 2, from Mary :: is gone at this time  
v; MKRS / Tm2   From Yikang, never used, bal. 3 :: may be gone  
       
       
       
  Box 2: yA and yB nuclease stocks, AB stocks, FLP+I-SceI stocks    
Name Genotype Comments  
Zif A on X, 2, and 3      
A6d v, [ry+, yA6d] A nuclease on X, never tested  
A16a v, [ry+, yA16a]/C(1)Dx A nuclease on X, never tested  
A5a v; [ry+, yA5a] / Sm6,Cyo; ry A nuclease on 2, only balanced, very good  
A2b v; [ry+, yA2b] A nuclease on 3, hz $, good  
A11a v; [ry+, yA11a] / Tm6,Ubx nuclease on 3, only balanced, very good  
Zif B on X, 2, and 3      
B1d v,[ry+, yB1d]; ry B nuclease on X, never tested  
B7a v; [ry+, yB7a]; ry B nuclease on 2, hz $, very good  
B12a v; [ry+, yB12a]; ry B nuclease on 2, hz $, very good  
B2b v; [ry+, yB2b] / Tm6,Ubx B nuclease on 3, hz live, good  
B4b v; [ry+,y B4b] / Tm6,Ubx B nuclease on 3, hz live, good  
Stocks with A and B on 2      
[AB1] v; [ry+, yA5a],[ry+, yB7a] / Sm6, Cyo; ry AB nucleases on 2, no hz flies  
[AB2] v; [ry+, yA5a],[ry+, yB12a] / Sm6, Cyo; ry AB nucleases on 2, no hz flies  
[AB3] v; [ry+, yA6b],[ry+, yB7a]/Sm6, Cyo; ry AB nucleases on 2, hz live  
[AB6] v; [ry+, yA12a],[ry+, yB12a] / Sm6, Cyo; ry AB nucleases on 2, hz live  
[AB7] v; [ry+, yA20b],[ry+, yB7a] / Sm6, Cyo; ry AB nucleases on 2, hz live  
Stocks with A and B on 3      
[AB10] v; [ry+, yA11a],[ry+, yB4b] / Tm6, Ubx AB nucleases on 3, hz live  
[AB11] v; [ry+, yA2b],[ry+, yB2b] / Tm6, Ubx AB nucleases on 3, hz live  
FLP+I-SceI on 2 hz $!      
W1118; FLP11 W1118; [ry+,70 FLP]11 / S2,Cyo FLP on 2, only balanced $  
FLP + I-SceI 2B on2 W1118; [ry+, 70 FLP]11[v+, I-SceI]2B hz $  
       
       
       
  Box 3: [AB] nuclease stocks with YM-1 and Yellow donor stocks    
Name Genotype Comments  
[AB] nuclease stocks on 2 with YM-1 donor      
[AB1]; [YM-1] W1118; [ry+, yA5a],[ry+, yB7a]/S2,Cyo; [w+, YM-1] AB nucleases on 2 with hz YM-1 donor on 3  
[AB2]; [YM-1] W1118; [ry+, yA5a],[ry+, yB12a] / S2,Cyo; [w+, YM-1] AB nucleases on 2 with hz YM-1 donor on 3  
[AB3]; [YM-1] W1118; [ry+, yA6b],[ry+, yB7a] / S2,Cyo; [w+, YM-1] AB nucleases on 2 (hz live) with hz YM-1 donor on 3  
[AB] stocks on 3 in W1118 background      
W1118, [AB11] W1118; [ry+, yA2b],[ry+, yB2b] / Tm6, Ubx AB nucleases on 3, hz live  
[AB] on 2 with YM-1 donor      
[AB3]; [YM-1] W1118; [ry+, yA6b],[ry+, yB7a] / S2,Cyo; [w+, YM-1] AB nucleases on 2 (hz live) with hz YM-1 donor on 3  
[AB7]; [YM-1] W1118; [ry+, yA20b],[ry+, yB7a] / S2,Cyo; [w+, YM-1] AB nucleases on 2 (hz live) with hz YM-1 donor on 3  
W1118; [AB10] W1118; [ry+, yA11a],[ry+, yB4b] AB nucleases on 3, hz $.  
[AB11] W1118; [AB11] / Tm6, Ubx AB nucleases on 3, hz live  
Yellow donor stocks      
YM-1 W1118; [w+,YM-1] donor on chromosome 3, hz $.  
[YM-1],Sb W1118; [w+,YM-1],Sb / Tm6,Ubx donor marked with Sb  
W1118; FLP + I-SceI W1118; [FLP +I-Sce1], [AB10]/MKRS FLP, I-Sce1, and AB10 on 3  
YM-1 W1118; [w+,YM-1] donor on chromosome 3, hz $. Second set  
W1118; Sco/S2,Cyo; [YM-1] W1118; Sco / S2,Cyo; [w+,YM-1] hz donor on 3, balanced chromosome 2  
FLP11; [YM-1] W1118; [ry+,70 FLP]11 / S2,Cyo; [w+,YM-1] FLP on 2 combined with hz YM-1 donor on 3 :: gone  
[FLP+I-Sce1]; [YM-1] W1118; [ry+,70 FLP]11[v+,70 I-SceI]2b; [w+,YM-1] hz FLP+I-SceI on 2 with hz YM-1 donor on 3 :: gone  
       
       
       
  Box 4: Useful Balancers and Various Stocks    
Name Genotype Comments  
w; Sco/CyO GFP w; Sco/CyO GFP AB nucleases on 2, no hz flies  
yw; MHC DTS/TM6, Sb, Tb yw; MHC DTS/TM6, Sb, Tb    
GFP balancer w; Sb/ TM3 P[Act5c-GFP, w+] John Roote 13-19  
w; H94 Gal 4/TM6 Tb w; H94 Gal 4/TM6 Tb    
cn bw sp Kan2 lz 3876/ MKRS cn bw sp Kan2 lz 3876/ MKRS    
st st    
In(2R) vgw, vgw INVw/ In(2R) vgw, vgw INVw/    
In(2L)Cy, In(2R)Cy, amosRoi-1cn2bw'or'sp2 In(2L)Cy, In(2R)Cy, amosRoi-1cn2bw'or'sp2    
sp ; st sp;st    
W1118; Xa/CyO; MKRS   fragile balancer  
W1118: CyO/ nab'b'nocSco1K stw3 ; MKRS/TM6B, Tb'   lost  
b;cn b;cn    
W1118: P[yM, w+] 8C      
W1118; P[yM, w+] 9B      
w; Sco/ CyO act GFP   lost  
FM7   lost  
W1118; P[yM, w+] N   on 2, was here but as of 8/6/04 isn't here now  
[AB7]; [YM-1]   requ. PCR to make certain YM-1 was still there, gone?  
Various Stocks      
v;ry v;ry backup stock  
W1118; Sco / S2,Cyo W1118; Sco / S2,Cyo Chromosome 2 balancer backup stock  
W1118; Sb / Tm6,Ubx W1118; eukor2,Sb / Tm6,Ubx Chromosome 3 balancer backup stock  
       
       
       
  Box 5:Rosy (Ry) stocks and BrownA (BwA) nuclease    
Name Genotype Comments  
ry26   BL#4466 rec'd 5.16.02  
mwh' ry506 e'   BL#2558 rec'd 5.16.02  
cu car ry'   BL#470 rec'd 5.16.02  
st' ry2;sv spa-pol   BL#2515 rec'd 5.16.02  
H{w+mc, pD2-3}Hop8 y' w*   BL#2080 rec'd 6.02  
W1118; p'[w+his2-GFP] G2A   BL#5941 rec'd 5.16.02  
TM2,rysc red'e/TM6B,,ryCB Tb'ca'e   BL#1700 rec'd 5.16.02  
y':w*;CyO,,H{w+mcPD2-3}Hop2.1/Bc'EgfrE1   BL#2078 rec'd 6.02  
T(2:3)CyO-TM9,CyO;TM9 I(3) DTS 4'/   BL#3705 rec'd 6.02  
In(2LR) bwv5 ds33K bwvi ; H'      
sc'2'ec'ct6   BL#101 rec'd 7/2002  
bwA 1D v; "bwA"1D, ry/TM6 Tb, ry hz viable fingers in inverse orientation 1.7.04  
bw8A R1 D v; bw8A R1 D; ry/TM6, Tb bw8A fingers in reverse orientation 1.25.04  
"bwA" FN 5A   fingers in inverse orientation 1.7.04  
       
       
       
  Box 6:BrownA (bwA) and Brown B (bwB) nucleases    
Name Genotype Comments  
bw8A16C/SM6   A nuclease on 2  
bw8A8C/SM6   in AB4  
bw8A6B/SM6a   A nuclease on 2 in AB1  
bw8A10A/MKRS   A nuclease on 3 In AB3  
bw8A11A/SM6a   A nuclease on 2 In AB3  
bwA15C/MKRS   A nuclease on 3  
bw8A9A/MKRS   A nuclease on 3  
bwA1A/MKRS   A nuclease on 3  
bw8B6B/(Tb)   B nuclease on 3 in AB10  
bw8B3B/(Tb)   B nuclease on 3  
bw8B1A/SM5   B nuclease in AB2  
bw8B7C/(SM6)   B nuclease on 2  
bwA4A   A nuclease on X  
bw8B2B/(SM6)   A nuclease on 2  
bw8B2A   B nuclease hz on 3  
bwA15B   A nuclease on X  
       
       
bw8B4C/SM6   B nuclease on 2  
       
       
       
  Box 7:BrownAB (bwAB) nucleases and Brown B mutants    
Name Genotype Comments  
Df(2Rbw)S46/CyO bw      
Df(22)bw HB132, FRD/SM6a      
Pr Dr/TM3 Sb tld 10-      
P c'cn'bw'sp'/CyO      
y2 cv vf      
bwAB11-1 v;bwA10A,bwB3A AB nuclease on 3  
bwAB1 v;bwA6B,bwB1A    
bwAB3 v;bwA11A,bwB1E    
bwAB1-1 v;bwA6C,bwB1A AB nuclease on 2  
bwAB2 v;bwA6C,bwB1A AB nuclease on 2  
bwAB4 v;bwA8C,bwB1A AB nuclease on 2  
bwAB10 v;bwA13A,bwB6B AB nuclease on 3  
bwm12A/SM6   on 2  
bwm2A/MKRS   on 3  
bwm5B   on X primarily has white eyes with some brown eyes  
bwm15A   bals on X  
bwm13A/SM6   on 2  
bwm2D/SM6;MKRS   v eyes with pseudopupils  
bwm13A/SM6   on 2 bw,w, v eyes  
bwm22A/MKRS   on 3