Carroll - short CURRICULUM VITAE

I. PERSONAL DATA

• Name: Dana Carroll
• Birthdate: September 2, 1943
• Birthplace: Palm Springs , California USA
• Citizenship: United States
• Family Status: Married, 2 children

• Address: Department of Biochemistry
• University of Utah School of Medicine
• 15 N. Medical Drive East Rm 4520B
• Salt Lake City , Utah 84112-5650
• Telephone: 801-581-5977
• FAX: 801-581-7959
• E-mail: dana@biochem.utah.edu

II. EDUCATION

• B.A. 1965 Swarthmore College , Swarthmore , PA , Chemistry
• Ph.D. 1970 University of California , Berkeley , Biophysical Chemistry (thesis advisor: I. Tinoco, Jr.)
• Postdoctoral Fellow Beatson Institute for Cancer Research, Glasgow , Scotland 1970-1972 (advisor: John Paul)
• Postdoctoral Fellow Carnegie Institution of Washington, Department of
• 1972-1975 Embryology, Baltimore , MD (advisor: Donald D. Brown)

III. PROFESSIONAL EXPERIENCE

A. Full-time Positions:

• University of Utah , Department of Biochemistry
• Professor and Co-chairman 1985-1998
• Professor and Chairman 1998-present
• University of Utah , Department of Oncological Sciences (formerly Cellular, Viral and Molecular Biology; formerly Microbiology)
• Assistant Professor 1975-1981
• Associate Professor 1981-1988
• Professor 1988-2002

B. Part-time Positions:

• 1971-present Manuscript reviewer for:
  • Arch. Biochem. Biophys. Biochemistry
  • Biopolymers Cancer Res.
  • Cell Chromosoma
  • Cytogenetics and Cell Genetics Dev. Biol.
  • Eur. J. Biochem. Exptl. Cell Res.
  • Gene Genes to Cells
  • Genetics Genomics
  • Int. J. Dev. Biol. J. Biol. Chem.
  • J. Cell Biol. J. Exp. Zool.
  • J. Mol. Biol. Mol. Cell. Biol.
  • Nucleic Acids Res. Proc. Natl. Acad. Sci. USA
• 1975-1993 Adjunct faculty member, Department of Biology, University of Utah
• 1975-1982 Adjunct Assistant Professor
• 1982-1987 Adjunct Associate Professor
• 1987-1993 Adjunct Professor
• Jan.-June 1983 Visiting Fellow, Department of Biology, Yale University (with J.G. Gall)
• July-Sept. 1983 Visiting Scholar, MRC Mammalian Genome Unit, Edinburgh University , Scotland (with E.M. Southern)
• 1987-present Member, University of Utah Huntsman Cancer Institute
• 1993 External reviewer for proposed Ph.D. Program in Molecular, Cellular and Developmental Biology, Fred Hutchinson Cancer Research Center, State of Washington.
• 1993-1997 Member, Education Committee, Association of Medical and Graduate Departments of Biochemistry.
• June-July, 1995 Visiting Professor, Section de Recherche, Institut Curie, Paris , France .
• 2001-2002 External reviewer for Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine.

C. Grant Review Panels

• 1978-present External proposal reviewer for NSF, NIH, DOE, Human Frontier Science Organization.
• Jan. 1981 Ad hoc member, NIH Postdoctoral Fellowship Study Section
• Feb. 1982 Ad hoc member, NIH Molecular Biology Study Section
• Sept. 1987 Member, NIH Special Study Section, Molecular Cytology.
• 1988-1991 Member, Developmental Biology Review Panel, NSF
• 1994-1996 Member, Eukaryotic Genetics Review Panel, NSF
• Apr. 1997 Member, NIH Special Study Section, Genetics
• Oct. 2001 Member, NIH Special Study Section, Genetics
• Jan. 2002 Member, NCI Program Project Review Panel
• Feb. 2002 Ad hoc member, NIH Genome Study Section
• Jan. 2003 Member, NCI Program Project Review Panel
• Feb. 2003 Mail reviewer, NIH Medical Biochemistry Study Section
• June 2003 Ad hoc member, NIH Biochemistry Study Section

D. Current Research Awards

• 2001-2004 "Chimeric Nucleases for Gene Targeting in Drosophila," NIH Research Grant GM58504, Principal Investigator. Annual direct costs, $198,250.
• 2002-2005 "Stimulating Gene Targeting with Zinc Finger Nucleases," NIH Research Grant #GM/HD65173, Principal Investigator. Annual direct costs, $150,000.

IV. SCHOLASTIC HONORS

• Ivy Award (outstanding male student), Swarthmore College , 1965
• Sigma Xi, 1965
• Phi Beta Kappa, 1965
• USPHS Predoctoral Fellow, 1966-1970
• Jane Coffin Childs Postdoctoral Fellow, 1970-1972
• Carnegie Fellow, 1972-1973
• USPHS Postdoctoral Fellow, 1973-1975
• American Cancer Society Scholar in Cancer Research, 1983
• Visiting Professor, Institut Curie, 1995

V. MEMBERSHIP IN PROFESSIONAL SOCIETIES

• American Association for the Advancement of Science
• American Chemical Society
• American Society for Biochemistry and Molecular Biology
• American Society for Microbiology
• American Society of Gene Therapy
• Association of Medical and Graduate Departments of Biochemistry

VI. TEACHING RESPONSIBILITIES

• Advanced Molecular Biology
• Biophysical Chemistry
• Nucleic Acid Biochemistry
• Medical Biochemistry
• Genetic Engineering
• Molecular Biology for Clinical Investigators
• Biochemistry for Clinical Investigators
• Special Topics - Genetic Recombination, Transposable Elements, Oocyte Injection, DNA Repair

VII. BIBLIOGRAPHY

A. Original Publications

1. MacGillivray, A.J., Carroll, D., and Paul J., (1971). The heterogeneity of the non-histone chromatin proteins from mouse tissues. FEBS Lett. 13, 204-208.
2. Carroll, D. (1971). Continuous-flow salt gradient dialysis for the preparation of polynucleotide-polypeptide complexes. Anal. Biochem. 44, 496-502.
3. Carroll, D. (1972). Optical properties of deoxyribonucleic acid-polylysine complexes. Biochemistry 11, 421-426.
4. Carroll, D. (1972). Complexes of polylysine with polyuridylic acid and other polynucleotides. Biochemistry 11, 426-433.
5. Carroll, D., and Botchan, M.R. (1972). Competition between pentalysine and actinomycin D for binding to DNA. Biochem. Biophys. Res. Commun. 46, 1681-1687.
6. Wellauer, P.K., Reeder, R.H., Carroll, D., Brown, D.D., Deutch, A., Higashinakagawa, T., and Dawid, I.B. (1974). Amplified ribosomal DNA from Xenopus laevis has heterogenous spacer lengths. Proc. Natl. Acad. Sci. USA 71, 2823-2827.
7. Carroll, D., and Brown, D.D. (1976). Repeating units of Xenopus laevis oocyte-type 5S DNA are heterogeneous in length. Cell 7, 467-475.
8. Carroll, D., and Brown, D.D. (1976). Adjacent repeating units of Xenopus laevis 5S DNA can be heterogeneous in length. Cell 7, 477-486.
9. Brown, D.D., Carroll, D., and Brown, R.D. (1977). The isolation and characterization of a second oocyte 5S DNA from Xenopus laevis. Cell 12, 1045-1056.
10. O'Neill, F.J., and Carroll, D. (1978). Appearance of defective simian virus 40 following infection of cultured human glioblastoma cells. Virology 87, 109-119.
11. Carroll, D., and O'Neill, F.J. (1978). Genome maps of simian virus 40 defectives propagated in human glioblastoma cells. Virology 87, 120-129.
12. Humphries, S.E., Young, D., and Carroll, D. (1979). The chromatin structure of the 5S RNA genes of Xenopus laevis. Biochemistry 18, 3223-3231.
13. Carroll, D., Ajioka, R.S., and Georgopoulos, C. (1980). Bacteriophage cloning vehicles for studies of genetic recombination. Gene 10, 261-271.
14. Carroll, D., and Ajioka, R.S. (1980). Recombination of a eukaryotic DNA in bacteria. Gene 10, 273-281.
15. Carroll, D., Hansen, J.L., Maryon, E.B., and O'Neill, F.J. (1981). SV40 defectives selected during low multiplicity passage in A172 human glioblastoma cells. Virology 112, 461-471.
16. O'Neill, F.J., and Carroll, D. (1981). Amplification of papovavirus defectives during serial low multiplicity infections. Virology 112, 800-803.
17. O'Neill, F.J., Maryon, E.B., and Carroll, D., (1982). Isolation and characterization of defective simian virus 40 genomes which complement for infectivity. J. Virol. 43, 18-25.
18. Lam, B.S., and Carroll, D. (1983). Tandemly repeated DNA sequences from Xenopus laevis. I. Studies on sequence organization and variation in satellite 1 DNA (741 base-pair repeat). J. Mol. Biol. 165, 567-585.
19. Lam, B.S., and Carroll, D. (1983). Tandemly repeated DNA sequences from Xenopus laevis. II. Clusters of a 388 bp repeating unit. J. Mol. Biol. 165, 587-597.
20. Young, D., and Carroll, D. (1983). A regular arrangement of nucleosomes on 5S ribosomal genes in Xenopus laevis. Mol. Cell. Biol. 3, 720-730.
21. O'Neill, F.J., and Carroll, D. (1983). Role of defective simian virus 40 genomes in establishment and maintenance of persistently infected primate cell lines. Intervirology 19, 181-194.
22. Carroll, D. (1983). Genetic recombination of bacteriophage ??DNAs in Xenopus oocytes. Proc. Natl. Acad. Sci. USA 80, 6902-6906.
23. Carroll, D., Garrett, J.E., and Lam, B.S. (1984). Isolated clusters of paired tandemly repeated sequences in the Xenopus laevis genome. Mol. Cell. Biol. 4, 254-259.
24. Swindle, J., Ajioka, J., Dawson, D., Meyers, R., Carroll, D., and Georgopoulos, C. (1984). The nucleotide sequence of the Escherichia coli K12 nusB (groNB) gene. Nucl. Acids Res. 12, 4977-4985.
25. Carroll, D., Wright, S.H., Ajioka, R.S., and Hussey, Jr., C.E. (1984). Genetic recombination of Xenopus laevis 5S DNA in bacteria. J. Mol. Biol. 178, 155-172.
26. Garrett, J.E., and Carroll, D. (1986). Tx1: A transposable element from Xenopus laevis with some unusual properties. Mol. Cell. Biol. 6, 933-941.
27. Carroll, D., Wright, S.H., Wolff, R., Grzesiuk, E., and Maryon, E.B. (1986). Efficient homologous recombination of linear DNA substrates after injection into Xenopus laevis oocytes. Mol. Cell. Biol. 6, 2053-2061.
28. Grzesiuk, E., and Carroll, D. (1987). Recombination of DNAs in Xenopus oocytes based on short homologous overlaps. Nucl. Acids Res. 15, 971-985.
29. Garrett, J.E., Knutzon, D.S., and Carroll, D. (1989). Composite transposable elements in the Xenopus laevis genome. Mol. Cell. Biol. 9, 3018-3027.
30. Maryon, E.B., and Carroll, D. (1989). Degradation of linear DNA by a strand-specific exonuclease activity in Xenopus laevis oocytes. Mol. Cell. Biol. 9, 4862-4871.
31. Sweigert, S.E., and Carroll, D. (1990). Repair and recombination of X-irradiated plasmids in Xenopus laevis oocytes. Mol. Cell. Biol. 10, 5849-5856.
32. Urnes, M.S., and Carroll, D. (1990). Amylase synthesis as a simple model system for translation and hybrid arrest in Xenopus oocytes. Gene 95, 267-274.
33. Maryon, E., and Carroll, D. (1991). Involvement of single-stranded tails in homologous recombination of DNA injected into Xenopus laevis oocyte nuclei. Mol. Cell. Biol. 11, 3268-3277.
34. Maryon, E., and Carroll, D. (1991). Characterization of recombination intermediates from DNA injected into Xenopus laevis oocytes: Evidence for a nonconservative mechanism of homologous recombination. Mol. Cell. Biol. 11, 3278-3287.
35. Lehman, C.W., and Carroll, D. (1991). Homologous recombination catalyzed by a nuclear extract from Xenopus oocytes. Proc. Natl. Acad. Sci. USA 88, 10840-10844.
36. Jeong-Yu, S., and Carroll, D. (1992). Test of the double-strand-break repair model of recombination in Xenopus laevis oocytes. Mol. Cell. Biol. 12, 112-119.
37. Jeong-Yu, S., and Carroll, D. (1992). The effect of terminal nonhomologies on homologous recombination in Xenopus laevis oocytes. Mol. Cell. Biol. 12, 5426-5437.
38. Pont-Kingdon, G., Dawson , R.J. and Carroll, D. (1993). Intermediates in extrachromosomal homologous recombination in Xenopus laevis oocytes: Characterization by electron microscopy. EMBO J. 12, 23-34.
39. Lehman, C.W. and Carroll, D. (1993). Isolation of large quantities of functional, cytoplasm-free Xenopus laevis oocyte nuclei. Anal. Biochem. 211, 311-319.
40. Lehman, C.W., Clemens, M., Worthylake, D.K., Trautman, J.K. and Carroll, D. (1993). Homologous and illegitimate recombination in developing Xenopus oocytes and eggs. Mol. Cell. Biol. 13, 6897-6906.
41. Lehman, C.W., Trautman, J.K. and Carroll, D. (1994). Illegitimate recombination in Xenopus: Characterization of the end-joined junctions. Nucleic Acids Res. 22, 434-442.
42. Carroll, D., Lehman, C.W., Jeong-Yu, S., Dohrmann, P., Dawson , R.J. and Trautman, J.K. (1994). Distribution of exchanges upon homologous recombination of exogenous DNA in Xenopus laevis oocytes. Genetics 138, 445-457.
43. Lehman, C.W., Jeong-Yu, S., Trautman, J.K. and Carroll, D. (1994). Repair of heteroduplex DNA in Xenopus laevis oocytes. Genetics 138, 459-470.
44. Segal, D.J. and Carroll, D. (1995). Endonuclease-induced, targeted homologous extrachromosomal recombination in Xenopus oocytes. Proc. Natl. Acad. Sci. USA 92, 806-810.
45. Faruqi, A.F., Seidman, M.M., Segal, D.J., Carroll, D. and Glazer, P.M. (1996). Recombination induced by triple helix-targeted DNA damage in mammalian cells. Mol. Cell. Biol. 16, 6820-6828.
46. Pont-Kingdon, G., Chi, E., Christensen, S. and Carroll, D. (1997). Ribonucleoprotein formation by the ORF1 protein of the non-LTR retrotransposon Tx1L in Xenopus oocytes. Nucleic Acids Res. 25, 3088-3094.
47. Segal, D.J., Faruqi, A.F., Glazer, P.M. and Carroll, D. (1997). Processing of targeted psoralen crosslinks in Xenopus oocytes. Mol. Cell. Biol. 17, 6645-6652.
48. Bibikova, M., Wu, B., Chi, E., Kim, K.-H., Trautman, J.K. and Carroll, D. (1998). Characterization of FEN-1 from Xenopus laevis. cDNA cloning and role in DNA metabolism. J. Biol. Chem. 273: 34222-34229.
49. Pont-Kingdon, G., Chi, E., Christensen, S. and Carroll, D. (1998). RNA expression from a site-specific non-LTR retrotransposon microinjected into Xenopus oocytes. Genetica, 104: 67-76.
50. Faruqi, A.F., Datta, H.J., Carroll, D., Seidman, M.M. and Glazer, P.M. (2000). Triple helix formation induces recombination in mammalian cells via a nucleotide excision repair-dependent pathway. Mol. Cell. Biol. 20, 990-1000.
51. Christensen, S., Pont-Kingdon, G. and Carroll, D. (2000). Target specificity of the endonuclease from the Xenopus laevis non-LTR retrotransposon, Tx1L. Mol. Cell. Biol. 20, 1219-1226.
52. Smith, J., Bibikova, M., Whitby , F.G., Reddy, A.R., Chandrasegaran, S. and Carroll, D. (2000). Requirements for double-strand cleavage by chimeric restriction enzymes with zinc finger DNA-recognition domains. Nucleic Acids Res. 28: 3361-3369.
53. Bibikova, M., Carroll, D., Segal, D.J., Trautman, J.K., Smith, J., Kim, Y.-G. and Chandrasegaran, S. (2001). Stimulation of homologous recombination through targeted cleavage by a chimeric nuclease. Mol. Cell. Biol. 21, 289-297.
54. Costanzo, V., Robertson, K., Bibikova, M., Kim, E., Grieco, D., Gottesman, M., Carroll, D. and Gautier, J. (2001). Mre11 protein complex prevents double-strand break accumulation during chromosomal DNA replication. Mol. Cell 8: 137-147.
55. Christensen, S., Pont-Kingdon, G. and Carroll, D. (2001). Comparative studies of the endonucleases from two related Xenopus laevis Retrotransposons, Tx1L and Tx2L: Target site specificity and evolutionary implications. Genetica 110: 245-256.
56. Bibikova, M., Golic, M., Golic, K.G. and Carroll, D. (2002). Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases. Genetics 161: 1169-1175.
57. Bibikova, M., Beumer, K., Trautman, J.K. and Carroll, D. (2003). Enhancing gene targeting with designed zinc-finger nucleases. Science 300: 764.
58. Pont-Kingdon, G., Chi, E. and Carroll, D. An L1 retrotransposon from Xenopus laevis and a conserved coiled-coil motif in all L1 elements. Submitted for publication.

B. Book Chapters

1. Paul, J., Carroll, D., Gilmour, R.S., More, I.A.R., Threlfall, G., Wilkie, M., and Wilson , S. (1972). Functional studies on chromatin. In Gene Transcription in Reproductive Tissue, 5th Karolinska Symposium on Research Methods in Reproductive Endocrinology, pp. 277-295.
2. MacGillivray, A.J., Rickwood, D., Cameron, A., Carroll, D., Ingles, C.J., Krauze, R.J., and Paul, J. (1975). Methods of isolation and characterization of chromosomal non-histone proteins. Meth. Enzymol. 40, 160-171.
3. Carroll, D., Knutzon, D.S., and Garrett, J.E. (1989). Transposable Elements in Xenopus. In Mobile DNA, M.M. Howe and D.E. Berg, eds., ASM Press, pp. 567-574.
4. Carroll, D., and Lehman, C.W. (1991). DNA recombination and repair in oocytes, eggs and extracts. Chapter 24 in Xenopus laevis: Practical Uses in Cell and Molecular Biology, v. 36 of Methods in Cell Biology, B.K. Kay and H.B. Peng, eds., Academic Press, pp. 467-486.
5. Carroll, D. (1996). Homologous genetic recombination in Xenopus: Mechanism and implications for gene manipulation. In Progress in Nucleic Acid Research and Molecular Biology, W.E. Cohn and K. Moldave, eds., vol. 54, Academic Press, pp. 101-125.
6. Carroll, D. (1998). Modes of DNA repair in Xenopus oocytes, eggs and extracts. In DNA Damage and Repair -- Biochemistry, Genetics and Cell Biology, M.F. Hoekstra and J.A. Nickoloff, eds., vol. 1, DNA Repair in Prokaryotes and Lower Eukaryotes, Chapter 26, Humana Press, pp. 597-616.
7. Carroll, D. and Segal, D.J. (1998). Induced recombination in model systems. Current Research in Molecular Therapeutics, 1: 593-598.
8. Carroll, D. (1999). DNA recombination and repair in Xenopus oocytes and eggs: substrate design, direct microinjection, and extract preparation. In A Comparative Methods Approach to the Study of Oocytes and Embryos, J.D. Richter, ed., Oxford University Press, New York , pp. 173-195.
9. Carroll, D. (2001). Invited chapters on:
   1. DNA Recombination
   2. Gene Duplication
   3. Illegitimate Recombination
   4. rDNA Amplification
   5. Recombinant DNA
   6. Encyclopedia of Genetics, S. Brenner and J.H. Miller, eds. Academic Press, NY.

C. Book Reviews

1. Carroll, D. (1990). Review of Chromatin, by K.E. Van Holde. Am. Scientist 78, 276.
2. Carroll, D. (1993). Review of Mechanisms of Eukaryotic DNA Recombination, M.E. Gottesman and H.J. Vogel, eds. BioScience 43, 639-640.
3. Revised September, 2002