Dr. Winge's laboratory consists of four wet laboratories, one computer room and two equipment rooms. The wet laboratories contains usual biochemistry and molecular biology laboratory equipment. In addition, we have two FPLC units for protein purification.
Instrument rooms adjacent to the wet laboratories contain the following:
1. Spectrophotometer room: contains an atomic absorption spectrometer (Perkin-Elmer AAnalyst 100), ICP spectrometer (Perkin-Elmer 3100 XL Optima), fluorimeter (Perkin-Elmer 650-10S), two recording UV/VIS spectrophotometers (Beckman DU640 and DU65 units), lyophilizer, amino acid analyzer (Beckman 6500), Beckman System Gold 126 HPLC unit , gel boxes for DNA analyses and two PCR rapid cyclers.
2. Centrifuge room: contains two low speed centrifuges, two high speed centrifuges and one ultracentrifuge. In addition, two low temperature freezers are present.
3. Incubator room: contains multiple shaking incubators for yeast and bacterial culturing, French Press and X-Press for making cell extracts, sonicator, beta scintillation counter, gamma radiation counter, and a vacuum concentrator.
4. Glassware washing room: the automated facility for glassware washing.
5. Cold Room: contains chromatography equipment .
6. Dark Room: contains a gel imager, nearby are a Molecular Dynamics PhosphorImager and a Konica autoradiography developer.
Core Facilities: The University of Utah Health Sciences Center maintains a series of core facilities that the Winge laboratory uses. Relevant facilities include:
1. Protein/DNA Core Facility: located in an adjacent building is operated with three DNA oligonucleotide synthesizers, two Edman protein sequencers and a solid phase peptide synthesizer.
2. DNA Sequencing Facility: offers custom sequencing of single strand, double strand and plasmid DNA and the sequencing of PCR products.
3. Mass Spectrometer Facility: Core MS instruments include an electrospray MS (with HPLC on-line capability), electrospray ion trap, MALDI spectrometer, and a tandem MS/MS unit. This facility operates on a service for fee basis.
4. NMR Core Facility: Unity 500 MHz Varian spectrometer, Varian Inova 600 MHz instrument with triple resonance and pulse field gradient capabilities.
5. X-Ray Crystallography: Two X-ray rotating anode generators and RAXIS area detectors are available with cryo-coolers in the Department of Biochemistry under the direction of Dr. C.P. Hill. Three constant temperature rooms set at 4, 13, and 21 degree C are available for crystallization trials.
6. Protein Interaction Core: Contains three BIAcore 2000 unit for the study of macromolecular interactions. Dr. Myszka also oversees a titration calorimeter (MicroCal MC-2 ITC and DSC), circular dichroism spectrometer (AVIV 62DS), dynamic light scattering instrument, and an analytical ultracentrifuge for sedimentation equilibrium centrifugation.
For additional details on the core facilities, click here